Trisomy scRNA-seq analysis

Pipeline Stages

1. Seurat v1
2. Seurat v2
3. glmmTMB Volcano Plots
   • Full genome
   • chr21 removed
4. glmmTMB DE (interaction plots, ISG, top genes)
   • Full genome
   • chr21 removed
5. GSEA (glmmTMB, trisomy)
   • Full genome
   • chr21 removed
6. hdWGCNA (trisomy)
7. hdWGCNA + glmmTMB stats (trisomy)